Involvement of inhibition of nucleus GAPDH over-expression in erythropoietin's reduction of neuronal apoptosis induced by brain ischemia/reperfusion in rats.
- Author:
Yan-Zhong GUAN
1
;
Ran GUO
;
Hong NIAN
;
Xiu-Dong JIN
Author Information
1. Department of Physiology, Mudanjiang Medical University, Mudanjiang, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
Brain;
enzymology;
pathology;
Brain Ischemia;
pathology;
Erythropoietin;
pharmacology;
Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating);
metabolism;
Humans;
Rats;
Rats, Sprague-Dawley;
Recombinant Proteins;
pharmacology;
Reperfusion Injury;
pathology
- From:
Acta Physiologica Sinica
2012;64(3):269-274
- CountryChina
- Language:Chinese
-
Abstract:
To study whether recombinant human erythropoietin (rhEPO) reduces neuronal apoptosis through inhibiting over-expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in nucleus induced by brain ischemia/reperfusion in rats, 48 adult Sprague-Dawley rats were randomly divided into 3 groups: sham, saline and EPO groups. Animal models of brain ischemia/reperfusion were established by middle cerebral artery occlusion in rats. The effects of EPO on the sizes of ischemia tissue were observed by TTC staining. The over-expression of GAPDH in nucleus was detected by Hoechst-33258 and anti-GAPDH antibody double staining. The neuronal apoptosis in penumbral was detected by Nissl's staining and Hoechst-33258 immunofluorescence, respectively. The results showed that rhEPO treatment (3 000 U/kg, three times daily, i.p.) apparently reduced the sizes of infarct brain tissue in ischemia/reperfusion rats. rhEPO inhibited over-expression of GAPDH in nucleus of apoptotic neurons. In the meantime rhEPO decreased the number of apoptotic neurons in ischemia/reperfusion rats. These results suggest that rhEPO may induced reduction of neuronal apoptosis in penumbra may be through inhibiting over-expression of GAPDH in nucleus of apoptotic neurons induced by ischemia/reperfusion. Reduction of GAPDH over-expression in nucleus may play a pivotal role in EPO inhibiting neuronal apoptosis in cerebral ischemia/reperfusion rats, providing experimental evidence for EPO neuro-protecting effects against ischemia/reperfusion.
