Comparative study on specific chromatograms and main active components of wild and cultivated rhizomes of Paris polyphylla var. yunnanensis.
10.19540/j.cnki.cjcmm.20170512.006
- Author:
Ke ZAN
1
;
Yu-Ming GAO
2
;
Gan CUI
3
;
Jie LIU
1
;
Li-Nong GUO
1
;
Jian ZHENG
1
;
Shuang-Cheng MA
1
Author Information
1. National Institutes for Food and Drug Control, Beijing 100050, China.
2. Ziyang Institute for Food and Drug Control, Ziyang 641300, China.
3. School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China.
- Publication Type:Journal Article
- Keywords:
HPLC;
Paris polyphylla var. yunnanensis;
content determination;
cultivated;
specific chromatograms;
steroidal saponins;
wild
- From:
China Journal of Chinese Materia Medica
2017;42(15):3011-3016
- CountryChina
- Language:Chinese
-
Abstract:
The present study is to compare specific chromatograms and main acitive components between wild and cultivated rhizomes of Paris polyphylla var. yunnanensis by HPLC. HPLC analysis was performed on a Waters XSelect HSS T3 C₁₈ clumn (4.6 mm×250 mm, 5 μm), with a mobile phase consisting of acetonitrile (A)-water (B) at a flow rate of 1 mL•min⁻¹ (0-50 min,30%-50%A;50-80 min,50% A,80-85 min,50%-30%A;85-100 min,30% A). The detection wavelength was 203 nm and the column temperature was controlled at 30 ℃, and the injection volume was 10 μL. HPLC specific chromatograms of wild and cultivated rhizomes of P. polyphylla var. yunnanensis were established and nine steroidal saponins were simultaneously determined by the above method. The mean contents of paris saponin Ⅶ, paris saponin H and total average contents of four pennogenyl saponins in Rhizomes of wild samples were significantly higher than those of cultivated ones. However, this result is opposite from the average content of paris saponin Ⅰ and total average contents of five dioscins in the wild and cultivated samples. Because the significant differences occurred for the specific chromatograms and main active components between the wild and cultivated P. polyphylla var. yunnanensis, much more pharmacological and clinical researches are therefore necessary.
