Molecular cloning, bacterial expression analysis and functional characterization of pathogenesis-related PR10-2 gene in Panax notoginseng.
10.19540/j.cnki.cjcmm.20170714.007
- Author:
Dan YANG
1
;
Yi BAO
1
;
Li-Mei CHEN
1
;
Xiu-Ming CUI
1
;
Kun-Zhi LI
1
Author Information
1. Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China.
- Publication Type:Journal Article
- Keywords:
Panax notoginseng;
pathogenesis-related protein;
prokaryotic expression;
root rot disease
- From:
China Journal of Chinese Materia Medica
2017;42(16):3106-3111
- CountryChina
- Language:Chinese
-
Abstract:
Base on the transcriptome analysis and RT-PCR techniques,a pathogenesis-related protein 10 gene was isolated from Panax notoginseng root and named as PnPR10-2. Bioinformatics and phylogenetic trees analysis revealed that open reading frame (ORF) of PnPR10-2 was 465 bp in length,encoding 154 amino acids,containing one typical conserved domain of pathogenesis related protein Bet v I family, and showed high similarity with that from P. ginseng. The recombinant expressed plasmid pET32a(+)-PnPR10-2 was expressed in Escherichia coli BL21. The expression conditions were optimized and it could be expressed well in soluble and inclusion body protein. Purified PnPR10-2 recombinant protein from the supernatant of cells was used to analysis the pathogen resistance activity by paper method. The purified recombinant protein could inhibit typical root rot disease pathogen (Fusarium solani and Cylindrocarpon destructans）growth evidently, we conjecture that PnPR10-2 may participated in defense response of P. notoginseng resistance to root rot disease pathogen.
