Study of heterologous efficient synthesis of cucurbitadienol.
10.19540/j.cnki.cjcmm.20170714.003
- Author:
Shou-Lian LI
1
;
Dong WANG
2
;
Yi LIU
2
;
Ting-Ting LIN
3
;
Jin-Lei TANG
2
;
Er-Bing HUA
1
;
Xue-Li ZHANG
2
;
Zhu-Bo DAI
2
;
Lu-Qi HUANG
3
Author Information
1. College of Biotechnology, Tianjin University of Science & Technology, Tianjin 310018, China.
2. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
3. State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
- Publication Type:Journal Article
- Keywords:
Sacchromy cescerevisiae;
cucurbitadienol;
synthetic biology;
triterpenoids
- From:
China Journal of Chinese Materia Medica
2017;42(17):3326-3331
- CountryChina
- Language:Chinese
-
Abstract:
Cucurbitadienol has anti-inflammation, anti-cancer activities, and acts as a precursor of traditional Chinese medicine active ingredients mogroside and cucurbitacine. For construction of a Sacchromyces cerevisiae cell factory for production of cucurbitadienol, we firstly cloned a cucurbitadienol synthase (CBS) gene from Siraitia grosvenorii. Then, through heterologous expression of CBS in the triterpenoid chassis strain WD-2091, the engineered strain could produced 27.44 mg•L ⁻¹ cucurbitadienol, which was determined by GC-MS. Further regulation of CBS expression led to cucurbitadienol's titer increasing by 202.07% and reaching 82.89 mg•L ⁻¹ in the shake flask fermentation and 1 724.10 mg•L ⁻¹ in the high cell density fermentation. Our research promotes the cucurbitane-type tetracyclic triterpenoids synthesis pathway analysis progress and provides the basis for further obtaining cell factories for production of cucurbitadienol tetracyclic triterpenoids.
