HUVECs extraction and UHPLC LTQ Orbitrap analysis for screening vascular endothelium protective components in Kudiezi injection.
10.19540/j.cnki.cjcmm.20170801.001
- Author:
Si-Yi LIU
1
;
Hong-Zhu ZHANG
2
;
Jing FAN
3
;
Zhan-Peng SHANG
1
;
Fei WANG
1
;
Wei-Jie LI
1
;
Ying LIU
1
;
Jian-Qiu LU
1
;
Jia-Yu ZHANG
1
Author Information
1. Beijing University of Chinese Medicine, Beijing 100029, China.
2. The First Hospital of Fangshan District, Beijing 102400, China.
3. WuXi AppTec, Shanghai 200131, China.
- Publication Type:Journal Article
- Keywords:
HUVECs;
Kudiezi injection;
UHPLC LTQ orbitrap;
cell chromatography
- From:
China Journal of Chinese Materia Medica
2017;42(17):3362-3367
- CountryChina
- Language:Chinese
-
Abstract:
An effective method has been employed as a tool for screening active components in Kudiezi injection by using cell chromatography and sensitive UHPLC-HR-MSn method. The potential bioactive components in Kudiezi injection could be selectively bound to the HUVECs target cells first. After cell target desensitization and inactivation, the chemical constituents with cell target affinity were identified by LC-MS, so as to screen the possible active components in Kudiezi injection. Based on the accurate mass measurements and the retention time, in total, 9 compounds were tentatively identified and characterized, including 4 sesquiterpene lactones, 3 phenolic acids and 2 flavonoids. HUVECs biospecific extraction coupled with UHPLC-LTQ-Orbitrap analysis could provide a rapid and efficient method for the identification of potential bioactive components in Kudiezi injection, and provide the reference for further research on its effective materials basis.
