Antifungal effects of three medicinal crops on Phytophthora nicotianae.
10.19540/j.cnki.cjcmm.20170814.010
- Author:
Da-Min HE
1
;
Yang CHEN
1
;
Shui-Ping YANG
1
;
Xue ZHANG
2
;
Jian ZHAO
3
;
Jing-Jing MO
3
;
Dong-Yan ZHANG
1
;
Xin-Mei ZHAO
1
;
Da-Xia CHEN
4
;
Wei DING
1
Author Information
1. College of Resources and Environment, Southwest University, Chongqing 400715, China.
2. Chongqing Medical and Pharmaceutical College, Chongqing 401331, China.
3. Zunyi Subsidiary of Guizhou Tobacco Company, Zunyi 563000, China.
4. Chongqing Academy of Chinese Material Medica, Chongqing 400065, China.
- Publication Type:Journal Article
- Keywords:
Chuanmingshen violaceum;
Phytophthora nicotianae;
Scrophularia ningpoensis;
antifungal;
extract;
root exudate
- From:
China Journal of Chinese Materia Medica
2017;42(18):3509-3515
- CountryChina
- Language:Chinese
-
Abstract:
Tobacco black shank is one of the most harmful soil-borne diseases infected by Phytophthora parasitica. In order to probe the control method to this disease, in this study, the mycelial growth rate method was employed to investigate the antifungal effects of extracts from stem-leaf and root, root exudates, and their combination of Scrophularia ningpoensis, Chuanmingshen violaceum and Pinellia ternata The results showed that: ①Stem-leaf and root extracts of S. ningpoensis, C. violaceum and P. ternata exhibited different antifungal activities, and the inhibition increased with the increase of extract concentration. The antifungal effect of S. ningpoensis extracts at 0.5 g•mL⁻¹ was the strongest than other medicinal plants, the inhibition rate of steam-leaf and root extracts reached 74.88%, 69.27%, respectively. The inhibitory effect of C. violaceum and P. ternata was relatively lower, however, there is a significant gain effect after combination of steam-leaf and root extracts of C. violaceum. ②The root exudates of S. ningpoensis, C. violaceum and P. ternata showed fungistasis to Phytophthora nicotianae, and fungistasis was enhanced with the increase of root exudate concentration. The antifungal effect in the order of C. violaceum > S. ningpoensis > P. ternata. ③The antifungal activity of combination of extract and root exudate from S. ningpoensis was similar with the effect of C. violaceum, they were both stronger than P. ternata, and the antifungal activity for three combination were located between the antifungal activity of their extracts and root exudates. S. ningpoensis and C. violaceum can be potentially applied to prevent and control the tobacco black shank.
