Standard decoction of Leonuri Herba.
10.19540/j.cnki.cjcmm.20170728.001
- Author:
Zhe DENG
1
;
Fan ZHANG
2
;
Jun ZHANG
1
;
Qing ZHANG
1
;
Meng-Jiao JIAO
1
;
Guo-Yuan ZHANG
1
;
Jin-Tang CHENG
1
;
An LIU
1
;
Yue-Sheng WANG
1
Author Information
1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
2. Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
- Publication Type:Journal Article
- Keywords:
Leonuri Herba;
fingerprint;
leonurine hydrochloride;
stachydrine hydrochloride;
standard decoction
- From:
China Journal of Chinese Materia Medica
2017;42(18):3523-3529
- CountryChina
- Language:Chinese
-
Abstract:
To build an evaluation standard for quality of Leonuri Herba standard decoction. 13 batches of Leonuri Herba standard decoction with different quality were prepared. The contents of leonurine hydrochloride and stachydrine hydrochloride were determined; then the transfer rate and the extract rate were calculated and pH value was measured; and HPLC fingerprint method was established for analysis. The results of 13 batches of samples revealed that the transfer rate of leonurine hydrochloride and stachydrine hydrochloride was 30.0%-53.4% and 67.0%-82.6%, respectively; the extract rate was 12.1%-18.3% and the pH value was 5.87-6.22. Moreover, 12 common chromatographic peaks were determined based on fingerprint by using Similarity Evaluation System for Chromatographic fingerprint of Traditional Chinese Medicine (2012A). The similarity of 13 batches of samples was analyzed and compared, and the results showed that the similarity was higher than 0.9. In this study, the preparation method for Leonuri Herba decoction was standard, with high similarity in fingerprint, showing high precision, stability and repeatability in fingerprint analysis. Thus, this study can provide a reference for the quality control of Leonuri Herba dispensing granules.
