Analysis of alcohol extract and water extract of Polygonum capitatum by UPLC-Q-TOF-MS.
10.19540/j.cnki.cjcmm.20170728.004
- Author:
Wen ZHOU
1
;
Li-Yan ZHANG
1
;
Li-Min XIE
2
;
Li-Fang QIU
2
;
Jing-Rong WANG
2
;
Si-Bu MA
1
;
Zhi-Hong JIANG
2
;
Jing-Wen TANG
3
Author Information
1. School of Pharmacy, Guiyang College of Traditional Chinese Medicine, Guiyang 550002, China.
2. State Key Laboratory of Quality of Traditional Chinese Medicine, Macao University of Science and Technology, Macau 999078, China.
3. Guizhou Weimen Pharmaceutical Co., Ltd., Guiyang 550004, China.
- Publication Type:Journal Article
- Keywords:
Polygonum capitatum;
UPLC-TOF-MS;
chemical composition;
extract
- From:
China Journal of Chinese Materia Medica
2017;42(18):3557-3563
- CountryChina
- Language:Chinese
-
Abstract:
In this study, we used Ultra Performance Liquid Chromatography-Time-of-Flight Mass Spectrometry(UPLC-TOF-MS)to identify the chemical constituents in both ethanol and water extract of Polygonum capitatum. A Waters ACQUITY UPLC BEH C₁₈ column(2.1 mm×100 mm,1.7 μm) was used for separation. The mobile phase was consisted of(A) 0.10% formic acid in water and(B)0.10% formic acid in acetonitrile, and the flow rate was 0.35 mL•min⁻¹. ESI source in negative ion mode was used for MS detection. Structural identification was carried out according to the accurate mass and matching with database. The results showed that flavonoids, polyphenols and lignans were the main components in both extracts. However, the chemical compositions of both extracts were different, e.g. there are less hydrolyzable tannins, loss of ellagic acid and more anthocyanins in ethanol extract. In a conclusion, this study provides an important scientific basis for identifying the active ingredients in P. capitatum, which also help to reveal the pharmacological effect of P. capitatum.
