Targets identification and mechanism analysis for macrophage activation of low molecular weight saccharides from Cistanche deserticola based on molecular affinity chromatography.
10.19540/j.cnki.cjcmm.2017.0145
- Author:
Li-Chao WANG
1
;
Dan LIU
2
;
Yong JIANG
1
;
Peng-Fei TU
1
;
Ke-Wu ZENG
1
Author Information
1. State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
2. Proteomics Laboratory, Medical and Health Analysis Center, Peking University, Beijing 100191, China.
- Publication Type:Journal Article
- Keywords:
Cistanche deserticola;
low molecular weight saccharides;
macrophage activation;
mechanism analysis;
molecular affinity chromatography;
target identification
- From:
China Journal of Chinese Materia Medica
2017;42(19):3666-3671
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to investigate the targets and targets-involved mechanism for the macrophage activation of low molecular weight saccharides from Cistanche deserticola (LMSC). The phagocytic activity and NO release of RAW264.7 cells were detected, and results showed that LMSC exerts immune activation effect by significantly increasing the phagocytic activity and NO release. LMSC-conjugated epoxy-activated sepharose beads were prepared as affinity reagent to capture the target proteins. Twenty-four proteins such as Eef2 were identified by LC-MS/MS analysis. Pathway enrichment analysis showed LMSC activated RAW264.7 cells by regulating Fcgamma receptor dependent phagocytosis, TNF-alpha NF-κB signaling pathway, glycolysis/gluconeogenesis and the citric acid cycle and respiratory electron transport pathway.
