Study on calibration standard for reference drug of Lonicera japonica cultivated in Xinmi.
10.19540/j.cnki.cjcmm.2017.0146
- Author:
Jian YANG
1
;
Chao JIANG
1
;
Yan JIN
1
;
Yu-Yang ZHAO
1
;
Yuan YUAN
1
;
Lu-Qi HUANG
1
Author Information
1. State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
- Publication Type:Journal Article
- Keywords:
Dao-di herbs;
Lonicera japonica;
Mi Yin Hua;
fingerprint
- From:
China Journal of Chinese Materia Medica
2017;42(19):3718-3722
- CountryChina
- Language:Chinese
-
Abstract:
The calibration technical specification for reference drug of Lonicera japonica cultivated in Xinmi (Mi Yin Hua) established in this study can be used as the theoretical basis and technical standard for internal quality control and evaluation of reference drug of Mi Yin Hua and for modern research and characteristics identification of Dao-di herbs. Based on the quality standard of L. japonica in Chinese Pharmacopoeia (version 2015), 10 batches samples of Mi Yin Hua also conformed to the stipulation. The LC-MS fingerprint common mode of Mi Yin Hua was obtained, and a total of 23 characteristic peaks were selected as the common fingerprint peaks, and eight common chromatographic peaks in the fingerprint was identified based on standard substances and references. The similarities of 10 batches samples were greater than 0.95. Seven core primers were used to construct SSR fingerprint and the ten batches samples were only 0 or 1 site disaccord with the standard diagram of Jianshan Damaohua germplasm from Xinmi. This paper constructed the first calibration standard of Dao-di herbals by combination of macroscopical identification, SSR fingerprint and LC-MS fingerprint, and the calibration standard provide theoretical basis and technical standard for evaluation system of Dao-di herbals.
