Chemical constituents from petroleum ether fraction of Swertia chirayita and their activities in vitro.
10.19540/j.cnki.cjcmm.20170807.003
- Author:
Rong-Rong YOU
1
;
Xue-Qing CHEN
2
;
Dan-Dan HE
1
;
Chang-Gao HUANG
3
;
Yang JIN
3
;
Shi-Hui QIAN
1
;
Jian-Ming JU
1
;
Jun-Ting FAN
3
Author Information
1. Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, China.
2. School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230031, China.
3. School of Pharmacy, Nanjing Medical University, Nanjing 211116, China.
- Publication Type:Journal Article
- Keywords:
Swertia chirayita;
chemical constituents;
oxidative stress damage;
pancreatic cancer cells;
petroleum ether fraction;
xanthone
- From:
China Journal of Chinese Materia Medica
2017;42(19):3764-3769
- CountryChina
- Language:Chinese
-
Abstract:
The present work is to study the chemical constituents from petroleum ether fraction of Tibetan medicine Swertia chirayita by column chromatography and recrystallization. The structures were identified by physical and chemical properties and spectral data as swerchirin (1), decussatin (2), 1,8-dihydroxy-3,5,7-trimethoxyxanthone (3), 1-hydroxy-3,5,7,8-tetramethoxyxanthone (4), bellidifolin (5), 1-hydroxy-3, 7-dimethoxyxanthone (6), methylswertianin (7), 1-hydroxy-3,5-dimethoxyxanthone (8), erythrodiol (9), oleanolic acid (10), gnetiolactone (11), scopoletin (12), sinapaldehyde (13), syringaldehyde (14), and β-sitosterol (15). Compounds 3, 4, 9, 11-14 were isolated from S. chirayita for the first time. Compounds 9 and 12 were firstly isolated from the genus Swertia. The cytotoxic activities of compounds 1, 2, 5, 7 and 8 against human pancreatic cancer cell lines SW1990 and BxPC-3,and the protective effects of these compounds against hydrogen peroxide (H2O2)-induced oxidative stress in human endothelium-derived EA.hy926 were investigated in vitro. The results showed no obvious effect at the high concentration of 50 μmol•L⁻¹.
