Simultaneous determination of sixteen mycotoxins contaminants in cogon rootstalk by QuEChERS-UPLC-QqQ mass spectrometry.
10.19540/j.cnki.cjcmm.20170905.001
- Author:
Miao-Xuan FAN
1
;
Jiao-Jiao DONG
2
;
Jing-Hui WANG
1
;
Hong-Zhu GUO
1
;
You-Gen CHEN
1
;
Xin-Tong FU
1
Author Information
1. Beijing Key Laboratory of Analysis and Evaluation on Chinese Medicine, Beijing Institute of Drug Control, Beijing 102206, China.
2. School of Chinese Materia Medica, Bejing University of Chinese Medicine, Beijing 100029, China.
- Publication Type:Journal Article
- Keywords:
QuEChERS;
UPLC-QqQ-MS/MS;
cogon rootstalk;
mycotoxin
- From:
China Journal of Chinese Materia Medica
2017;42(19):3770-3775
- CountryChina
- Language:Chinese
-
Abstract:
A method for the simultaneous determination of sixteen mycotoxins in cogon rootstalk was developed using ultra-performance liquid chromatography coupled with triple quadropole mass spectrometry(UPLC-QqQ-MS/MS). The samples were extracted with acetonitrile contained 1% acetic acid and purified by QuEChERS method. The separation was performed on an Agilent Eclipse Plus C₁₈column by gradient elution using methanol and 0.01% aqueous formic acid as mobile phase. The targeted compounds were detected in MRM mode by mass spectrometry with electrospray ionization(ESI)source operated in positive ionization mode. The linear relationships of the sixteen mycotoxins were good in their respective linear ranges. The correlation coefficients(r)ranged among 0.996 2-1.000. The LOQs of the sixteen mycotoxins were between 0.03 and 186.68 μg•kg ⁻¹. The average recoveries ranged from 60.28% to 129.2% with relative standard deviations(RSDs)within 0.29%-11%. The results demonstrated that the proposed method was sensitive and accurate, and suitable for the mycotoxins quantification in cogon rootstalk.
