Effects of sampling time on polysaccharides of Dendrobium catenatum under temperature-controlled condition.
10.19540/j.cnki.cjcmm.2017.0155
- Author:
Qi WANG
1
;
Jing-Jing LIU
1
;
Jin-Ping SI
1
;
Yu-Qiu ZHU
1
;
Lang QIN
2
;
Ju-Ling ZHANG
3
Author Information
1. State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin'an 311300, China.
2. Anhui Mulongshan Ecotourism Development Co., Ltd., Chuzhou 239500, China.
3. Ningbo Academy of Agriculture Sciences, Ningbo 315040, China.
- Publication Type:Journal Article
- Keywords:
Dendrobium catenatum;
culture time;
monosaccharide composition;
polysaccharides;
sampling time
- From:
China Journal of Chinese Materia Medica
2017;42(20):3891-3894
- CountryChina
- Language:Chinese
-
Abstract:
In order to reveal the accumulation trend of polysaccharides in Dendrobium catenatum and determine the effect of sampling time on polysaccharides, D. Catenatum D21 clone was harvested from January to December after culturing for 2 to 5 months in the growth chamber with constant temperature. Polysaccharides were determined by phenol-sulfuric acid method and the monosaccharide compositions were analyzed by pre-column derivative-UPLC. The results showed that the content of polysaccharide and its key component mannose was positively correlated with the culture time, but the contents of polysaccharides in all kinds of culture peaked from 5 to 6 months, which were consistent with the trend of field planting. The results suggested that the trend of polysaccharide accumulation in the plant could be related to the life rhythm of the sensory seasons of D. catenatum, which was significantly affected by the harvesting season, even under the constant condition of the culture chamber.
