Adsorption characteristics of proteins on membrane surface and effect of protein solution environment on permeation behavior of berberine.
10.19540/j.cnki.cjcmm.20170913.001
- Author:
Yi-Qun LI
1
;
Li XU
1
;
Hua-Xu ZHU
1
;
Zhi-Shu TANG
2
;
Bo LI
1
;
Yong-Lan PAN
1
;
Wei-Wei YAO
1
;
Ting-Ming FU
1
;
Li-Wei GUO
1
Author Information
1. Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, China.
2. Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Shaanxi University of Chinese Medicine, Xianyang 712046, China.
- Publication Type:Journal Article
- Keywords:
adsorption characteristics;
berberine;
ceramic membrane microfiltration;
protein;
simulation system
- From:
China Journal of Chinese Materia Medica
2017;42(20):3912-3918
- CountryChina
- Language:Chinese
-
Abstract:
In order to explore the adsorption characteristics of proteins on the membrane surface and the effect of protein solution environment on the permeation behavior of berberine, berberine and proteins were used as the research object to prepare simulated solution. Low field NMR, static adsorption experiment and membrane separation experiment were used to study the interaction between the proteins and ceramic membrane or between the proteins and berberine. The static adsorption capacity of proteins, membrane relative flux, rejection rate of proteins, transmittance rate of berberine and the adsorption rate of proteins and berberine were used as the evaluation index. Meanwhile, the membrane resistance distribution, the particle size distribution and the scanning electron microscope (SEM) were determined to investigate the adsorption characteristics of proteins on ceramic membrane and the effect on membrane separation process of berberine. The results showed that the ceramic membrane could adsorb the proteins and the adsorption model was consistent with Langmuir adsorption model. In simulating the membrane separation process, proteins were the main factor to cause membrane fouling. However, when the concentration of proteins was 1 g•L⁻¹, the proteins had no significant effect on membrane separation process of berberine.
