Influence of different cultivation measures on the content of three free carbohydrates of Codonopsis Radix.
10.19540/j.cnki.cjcmm.20170807.001
- Author:
Ting-Ting CAO
1
;
Jiu-Shi LIU
1
;
Shi-Man GAO
1
;
Tian SUN
2
;
Jie-Yuan QIN
2
;
Hao JING
3
;
Yao-Dong QI
1
;
Ben-Gang ZHANG
1
;
Hai-Tao LIU
1
;
Pei-Gen XIAO
1
Author Information
1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.
2. Infinitus (China) Company Ltd., Guangzhou 510623, China.
3. Shunxinghe (Min county) Traditional Chinese Medicine Company Ltd., Dingxi 743000, China.
- Publication Type:Journal Article
- Keywords:
Codonopsis Radix;
cultivation measures;
free carbohydrates;
quantitative analysis
- From:
China Journal of Chinese Materia Medica
2017;42(20):3963-3968
- CountryChina
- Language:Chinese
-
Abstract:
To provide a scientific evidence for the quality control of Codonopsis Radix, a method was established for determining the content of three free carbohydrates of Codonopsis Radix. The developed method showed good linearity. The calibration curves were linear within the range of 2.312 5-18.500 0 μg for sucrose, 1.500 0-12.000 0 μg for glucose, and 2.000 0-16.000 0 μg for fructose, resgectwely. The recoveries varied between 96.31%-101.8%. The method is simple, accurate and reproducible, and can be used for determining the content of sucrose, glucose and fructose of Codonopsis Radix. The results showed that different cultivation measures had an effect on the content of three free carbohydrates of Codonopsis Radix. According to the content of sucrose, using Zhuanggenling>not using Zhuanggenling. While, not pinching, shelving>not pinching, not shelving>pinching, not shelving>pinching, shelving. According to the content of glucose and fructose, not using Zhuanggenling>using Zhuanggenling. While, pinching, shelving>not pinching, not shelving>not pinching, shelving>pinching, not shelving. In consideration of the differences of sweetness and content of the three free carbohydrates in Codonopsis Radix, we recommend that the content of free carbohydrates could be considered as the marker to evaluate the quality of Codonopsis Radix.
