Loop-mediated isothermal amplification assay for the detection of Salmonella spp. in pig feces.
- Author:
Yong Kwan KIM
1
;
Ha Young KIM
;
Albert Byungyun JEON
;
Myoung Heon LEE
;
You Chan BAE
;
Jae Won BYUN
Author Information
1. Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency, Anyang 430-757, Korea. jaewon8911@korea.kr
- Publication Type:Brief Communication
- Keywords:
detection limit;
invA;
loop-mediated isothermal amplification assay;
Salmonella
- MeSH:
Animals;
Diagnosis;
Feces*;
Gastroenteritis;
Limit of Detection;
Salmonella Infections;
Salmonella*
- From:Korean Journal of Veterinary Research
2014;54(2):113-115
- CountryRepublic of Korea
- Language:English
-
Abstract:
Salmonella are causative agents of gastroenteritis and systemic disease in animals. The invA gene was selected as a target sequence of loop-mediated isothermal amplification (LAMP) assay for diagnosis of Salmonella infection. The detection limits for broth dilution, spiked feces and enrichment were 10(4), 10(5) and 10(2) CFUs/mL, respectively. The LAMP assay developed in the present study may be a reliable method for detection of Salmonella spp. in pig feces.
