Curcumin induces apoptosis through the mitochondria-mediated apoptotic pathway in HT-29 cells.
- Author:
Jin-bo WANG
1
;
Li-li QI
;
Shui-di ZHENG
;
Tian-xing WU
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents; pharmacology; Apoptosis; drug effects; Caspase 3; genetics; metabolism; Cell Nucleus; drug effects; Cell Survival; drug effects; Curcumin; pharmacology; Cytochromes c; secretion; HT29 Cells; Humans; Inhibitor of Apoptosis Proteins; Membrane Potential, Mitochondrial; drug effects; Microtubule-Associated Proteins; genetics; Mitochondria; physiology; Proto-Oncogene Proteins c-bcl-2; genetics; RNA, Messenger; analysis; bcl-2-Associated X Protein; genetics; bcl-X Protein; genetics
- From: Journal of Zhejiang University. Science. B 2009;10(2):93-102
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the effects of curcumin on release of cytochrome c and expressions of Bcl-2, Bax, Bad, Bcl-xL, caspase-3, poly ADP-ribose polymerase (PARP), and survivin of HT-29 cells.
METHODSHT-29 cells were treated with curcumin (0 approximately 80 micromol/L) for 24 h. The release of cytochrome c from the mitochondria and the apoptosis-related proteins Bax, Bcl-2, Bcl-xL, Bad, caspase-3, PARP, and survivin were determined by Western blot analysis and their mRNA expressions by reverse transcriptase-polymerase chain reaction (RT-PCR).
RESULTSCurcumin significantly induced the growth inhibition and apoptosis of HT-29 cells. A decrease in expressions of Bcl-2, Bcl-xL and survivin was observed after exposure to 10 approximately 80 micromol/L curcumin, while the levels of Bax and Bad increased in the curcumin-treated cells. Curcumin also induced the release of cytochrome c, the activation of caspase-3, and the cleavage of PARP in a dose-dependent manner.
CONCLUSIONThese data suggest that curcumin induced the HT-29 cell apoptosis possibly via the mitochondria-mediated pathway.
