Anti-angiogenesis effect of generation 4 polyamidoamine/vascular endothelial growth factor antisense oligodeoxynucleotide on breast cancer in vitro.
- Author:
Shan-zhi GU
1
;
Xin-han ZHAO
;
Ling-xiao ZHANG
;
Li LI
;
Zhi-yu WANG
;
Min MENG
;
Gai-li AN
Author Information
- Publication Type:Journal Article
- MeSH: Angiogenesis Inhibitors; genetics; Breast Neoplasms; blood supply; genetics; metabolism; pathology; Cell Line, Tumor; Cell Proliferation; drug effects; Dendrimers; Gene Expression Regulation; drug effects; Humans; Hydrogen-Ion Concentration; Microscopy, Electron, Transmission; Nylons; Oligodeoxyribonucleotides, Antisense; genetics; pharmacology; ultrastructure; RNA, Messenger; genetics; Transgenes; genetics; Vascular Endothelial Growth Factor A; genetics; metabolism; ultrastructure
- From: Journal of Zhejiang University. Science. B 2009;10(3):159-167
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study the effects of the generation 4 polyamidoamine/vascular endothelial growth factor antisense oligodeoxynucleotide (G4PAMAM/VEGFASODN) compound on the expressions of vascular endothelial growth factor (VEGF) and its mRNA of breast cancer cells and on the inhibition of vascular endothelial cells.
METHODSWe examined the morphology of G4PAMAM/VEGFASODN compound and its pH stability, in vitro transfection efficiency and toxicity, and the expressions of VEGF and its mRNA. Methyl thiazolyl tetrazolium assay was used to detect the inhibitory function of the compound on vascular endothelial cells.
RESULTSThe compound was about 10 nm in diameter and was homogeneously netlike. From pH 5 to 10, it showed quite a buffered ability. The 48-h transfection rate in the charge ratio of 1:40 was 98.76%, significantly higher than that of the liposome group (P<0.05). None of the transfection products showed obvious toxicity on the cells. The expressions of both VEGF protein and its mRNA after G4PAMAM/VEGFASODN transfection decreased markedly.
CONCLUSIONWith a low toxicity, high safety, and high transfection rate, G4PAMAM/VEGFASODN could be a promising gene vector. Specifically, it inhibits VEGF gene expression efficiently, laying a basis for further in vivo animal studies.
