Aldehyde dehydrogenase 1 can be used as a new marker of cancer stem cells in laryngeal cancer cells in vitro.
- Author:
Xin JIN
1
;
Yan ZHAO
;
Jun QIAN
;
Jie TANG
;
Xiao-dong ZHAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Biomarkers, Tumor; metabolism; Cell Culture Techniques; Cell Differentiation; Cell Line, Tumor; Cell Proliferation; Cell Separation; Flow Cytometry; methods; Humans; Isoenzymes; metabolism; Laryngeal Neoplasms; enzymology; pathology; Male; Mice; Mice, Nude; Neoplasm Transplantation; Neoplastic Stem Cells; enzymology; Random Allocation; Retinal Dehydrogenase; metabolism; Tumor Burden
- From: Chinese Journal of Oncology 2011;33(12):900-904
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the expression of aldehyde dehydrogenase 1 in human laryngeal cancer cells in vitro, and to explore whether it can be used as a marker of stem cells in human laryngeal cancer.
METHODSFluorescence staining and flow cytometry were used to detect the expression of ALDH1 in a human laryngeal cancer Hep-2 cell line, and fluorescence activated cells sorting was used to separate ALDH1(br) cells. ALDH1 tumor cells were cultured and their ability of proliferation and differentiation was observed in vitro.
RESULTSThe expression of ALDH1 in Hep-2 cells was different. The number of cells highly expressing ALDH1 was 2.9% ± 0.6%. Compared with ALDH1(low) cells and unsorted cells, ALDH1(br) cells exhibited increased proliferation ability. In serum-containing RPM I1640 culture medium, the proportion of ALDH1(br) cells was decreasing as days passed. The percentage of ALDH1(br) cells decreased from 94.2% ± 3.8% to the level before sorting. The ALDH1(br) cells demonstrated enhanced tumorigenic ability in nude mice.
CONCLUSIONSIn the laryngeal cancer Hep-2 cell line, the highly ALDH1-expressing cells show a strong ability of differentiation, proliferation and tumorigenesis. It indicates that ALDH1 can be used as a new marker of stem cells of laryngeal cancer cells.
