New compound NL-608 (a nutlin analog) induces apoptosis in human breast cancer MCF-7 cells.
- Author:
Ya-Kun GE
1
;
Yuan-Xin ZHANG
;
Yun-Peng CHEN
;
Xu BAI
;
Wei SHI
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents; pharmacology; Apoptosis; drug effects; Caspase 3; metabolism; Caspase 8; metabolism; Caspase 9; metabolism; Cell Proliferation; drug effects; Dose-Response Relationship, Drug; Fas Ligand Protein; metabolism; Humans; Imidazoles; pharmacology; MCF-7 Cells; Piperazines; pharmacology; fas Receptor; metabolism
- From: Chinese Journal of Oncology 2012;34(2):100-103
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of NL-608 (a nutlin analog) on apoptosis induction in human breast cancer MCF-7 cells in vitro, and investigate the relevant molecular mechanism.
METHODSThe effect of NL-608 on proliferation of MCF-7 cells was determined by MTT assay. The apoptosis in MCF-7 cells was determined by flow cytometry with annexin V-FITC and PI. The activity of caspase 3, caspase 8 and caspase 9 was determined with caspase activity assay kit and Western blot, and the proteins of Fas and FasL were determined by Western blot.
RESULTSNL-608 showed a dose-dependent inhibitory effect on the proliferation of MCF-7 cells. It induced apoptosis in MCF-7 cells in a dose-dependent manner. The activity of caspase 3 and caspase 8 in MCF-7 cells was increased with the increasing concentration of NL-608, but caspase 9 had no changes. The proteins of Fas and FasL were increased in a dose-dependent manner.
CONCLUSIONNL-608 induces apoptosis in MCF-7 cells in vitro through inducing caspase 3 activity and death receptor-mediated signal pathway.
