Inhibitory effect of folic acid/polyamide-amine as a miR-7 vector on the growth of glioma in mice.
- Author:
Xiao-zhi LIU
1
;
Zhi-guo SU
;
Zhong-min JIANG
;
Gang LI
;
Jun SONG
;
Kai HUANG
;
Liang WANG
;
Lei CHEN
;
Zhen-lin LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; Brain Neoplasms; genetics; metabolism; pathology; Cell Line, Tumor; Dendrimers; chemistry; Folic Acid; chemistry; Genetic Therapy; methods; Glioma; genetics; metabolism; pathology; Humans; Male; Matrix Metalloproteinase 2; metabolism; Matrix Metalloproteinase 9; metabolism; Mice; Mice, Nude; MicroRNAs; genetics; metabolism; Nanoparticles; Neoplasm Transplantation; Proliferating Cell Nuclear Antigen; metabolism; Proto-Oncogene Proteins c-akt; metabolism; Receptor, Epidermal Growth Factor; metabolism; Thymectomy; Transfection
- From: Chinese Journal of Oncology 2012;34(5):325-330
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore if folic acid/polyamide-amine (FA/PAMAM) enhances the therapeutic effect of miR-7gene therapy for glioma in vivo.
METHODSThe miR-7 gene was transfected into U251 glioma cells by FA/PAMAM. The efficiency of gene transfection was assessed by fluorescence microscopy. The miR-7 level was detect by quantitative RT-PCR. Intracranial glioma models were established in thymectomized mice, and FA/PAMAM nanoparticles were transplanted into the tumors in situ 3 days later. The animal survival was recorded and the gross tumor volume and degree of edema were observed by MRI. Apoptosis in the glioma cells and expression of proliferating cell nuclear antigen (PCNA), matrix metalloproteinases 2 and 9 (MMP-2 and MMP-9) were assessed by immunohistochemistry, and EGFR and AKT-2 protein expression was detected by Western blot assay.
RESULTSCompared with the liposomes, the FA/PAMAM nanoparticles were more efficient to transfer miR-7 gene into U251 glioma cells, MRI showed that the tumor growth was much slower in the FA/PAMAM/miR-7 group, and the animal survival time was longer. The apoptosis rate was (5.3 ± 0.9)% in the control group, (11.4 ± 2.4)% in the liposome/miR-7 group, and (17.7 ± 3.7)% in the FA/PAMAM/miR-7 group. The immunohistochemical assay showed that the levels of PCNA, MMP-2 and MMP-9 protein in the FA/PAMAM/miR-7 group were (34.6 ± 5.4)%, (24.5 ± 4.1)%, (25.4 ± 5.1)%, respectively, significantly lower than those in the liposome/miR-7 group (49.3 ± 5.9)%, (31.7 ± 7.1)% and (39.4 ± 6.4)%, respectively, and those in the control group (57.3 ± 7.4)%, (45.4 ± 6.9)% and (55.1 ± 7.3)%, respectively (all P < 0.05). The expressions of EGFR and AKT-2 proteins were 1.09 ± 0.12 and 0.62 ± 0.10 in the control group, 0.63 ± 0.11 and 0.43 ± 0.07 in the liposome/miR-7 group, and significantly deceased (0.47 ± 0.09 and 0.31 ± 0.04, respectively) in the FA/PAMAM/miR-7 group (all P < 0.05).
CONCLUSIONCompared with the liposomes, FA/PAMAM can transfect miR-7 into glioma cells with a higher efficiency in vivo, makes a longer time of the drug action, and shows a certain inhibitory effect on the growth of glioma, therefore, might become a new drug targeting agent in gene therapy forglioma.
