the effects of insulin-like growth factor i (igf-i) on expression of vascular endothelial growth factor (vegf) mrna in mg-63 osteoblastlike cells.
- Author:
Je Duck SUH
1
;
Hoon MYUNG
;
Nara KANG
;
Pill Hoon CHOUNG
Author Information
1. Department of Oral and Maxillofacial Surgery, Seoul National University Boramae Hospital, Korea.
- Publication Type:Original Article
- Keywords:
Osteogenesis;
Angiogenesis;
Vascular endothelial growth factor;
Insulin-like growth factor I;
MG-63 cell line
- MeSH:
Blotting, Northern;
Cycloheximide;
Dactinomycin;
DNA;
Hydroxyurea;
Insulin-Like Growth Factor I*;
Osteogenesis;
RNA;
RNA, Messenger*;
Vascular Endothelial Growth Factor A*
- From:Journal of the Korean Association of Oral and Maxillofacial Surgeons
2005;31(5):363-369
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To determine the role of Insulin-like Growth Factor-I (IGF-I) in the regulation of Vascular Endothelial Growth Factor (VEGF) expression in MG-63 cells and then to find the mechanism b which this regulation occurs. MATERIALS AND METHODS: MG-63 cells were grown to confluence in 60-mm dishes. To determine the effects of IGF-I on expression of VEGF mRNA according to time and concentration, the cells were treated with 10 nM IGF-I, following isolation of total RNA and Northern blot analysis after 1, 2, 4, 8, 12, 24 hours and after 2 hours of treatment with 0.5, 2, 10, 25, 50 nM IGF-I respectively, isolation of total RNA and Northern blot analysis were followed. To determine the mechanism of action of IGF-I, inhibitors such as hydroxyurea (76.1 microgram/ml), actinomycin D (2.5 microgra/ml), cycloheximide (10 microgram/ml) were added 1 hour after treatment of 10 nM IGF-I. RESULTS: 1. the expression of VEGF mRNA was increased with treatment of IGF-I. 2. The expression of VEGF mRNA was increased according to time- and concentration dependent manner of IGF-I. 3. The effect of IGF-I was decreased by hydroxyuera, actinomycin D, but not by cycloheximide. CONCLUSION: IGF-I regulate the expression of VEGF mRNA in the level of DNA synthesis and transcription. These results could suggest that IGF-I plays an important role in angiogenesis in the process of new bone formation and remodeling.
