Study of polymorphisms of HLA class Ⅰ (-A, -B, -C) and class Ⅱ (DRB1, DQA1, DQB1, DPA1, DPB1) genes among ethnic Hans from Southern China.
- VernacularTitle:中国南方汉族人群HLA-Ⅰ、Ⅱ类八个位点等位基因多态性的研究
- Author:
Shizheng JIN
1
;
Hongyan ZOU
;
Jianxin ZHEN
;
Daming WANG
;
Liumei HE
;
Zhihui DENG
Author Information
- Publication Type:Journal Article
- MeSH: Alleles; Asian Continental Ancestry Group; genetics; China; Gene Frequency; Genotype; Genotyping Techniques; methods; HLA-A Antigens; genetics; HLA-B Antigens; genetics; HLA-C Antigens; genetics; HLA-DP Antigens; genetics; HLA-DQ alpha-Chains; genetics; HLA-DQ beta-Chains; genetics; HLA-DRB1 Chains; genetics; Histocompatibility Antigens Class I; genetics; Histocompatibility Antigens Class II; genetics; Humans; Linkage Disequilibrium; Polymerase Chain Reaction; Polymorphism, Genetic
- From: Chinese Journal of Medical Genetics 2017;34(1):110-114
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the genetic polymorphisms of human leukocyte antigen (HLA)- A, B, C, DRB1, DQA1, DQB1, DPA1and DPB1among ethnic Hans from southern China.
METHODS481 randomly selected individuals were genotyped using a polymerase chain reaction (PCR) sequence-based typing (SBT) method for the above genes. Their allele frequencies were determined by direct counting.
RESULTSIn total, 28 HLA-A, 57 HLA-B, 28 HLA-C, 40 HLA-DRB1, 18 HLA-DQA1, 17 HLA-DQB1, 6 HLA-DPA1and 21 HLA-DPB1alleles were identified. Among these, common alleles (with allelic frequencies > 0.05) included A*1101, A*2402, A*0207, A*3303, A*0201, B*40:01, B*46:01, B*58:01, B*13:01, B*15:02, C*01:02, C*07:02, C*03:04, C*03:02, C*08:01, C*03:03, C*04:01, DRB1*09:01, DRB1*15:01, DRB1*12:02, DRB1*08:03, DRB1*03:01, DRB1*04:05, DRB1*11:01, DQA1*01:02, DQA1*03:02, DQA1*03:03, DQA1*06:01, DQA1*01:03, DQA1*05:05, DQA1*01:04, DQA1*03:01, DQA1*05:01, DQB1*03:01, DQB1*03:03, DQB1*06:01, DQB1*05:02, DQB1*03:02, DQB1*02:01, DQB1*03:02, DQB1*06:02, DPA1*02:02, DPA1*01:03, DPA1*02:01, DPB1*05:01, DPB1*02:01, DPB1*13:01, DPB1*04:01and DPB1*02:02.For each of the locus, the overall frequencies of common alleles were 75.57%, 52.81%, 78.28%, 62.16%, 86.70%, 77.23%, 95.32% and 81.59%, respectively.
CONCLUSIONThe allelic frequencies of the 8 selected HLA loci among ethnic Hans from southern China may served as a reference for anthropology, legal medicine, transplantation and disease association studies.
