Generation of GABAergic interneuron-specific PGC-1α knockout mice.
- VernacularTitle:γ-氨基丁酸能神经元条件性敲除PGC-1α基因小鼠的制备
- Author:
Jia WANG
1
;
Xianlu CHANG
;
Jinjun QIAN
;
Yang JIANG
;
Chunyan WANG
;
Yucong WANG
;
Chi FENG
;
Yongjie WANG
;
Peihui XIA
;
Ying ZHANG
;
Danping WU
;
Weining ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Female; Humans; Interneurons; metabolism; Male; Mice; Mice, Knockout; Neurodegenerative Diseases; genetics; Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; genetics; gamma-Aminobutyric Acid; metabolism
- From: Chinese Journal of Medical Genetics 2017;34(2):200-204
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo generate mice which are specific for peroxisomproliferator-activated receptor-γ coactivator-1(PGC-1α) knockout in the GABAergic interneuron.
METHODSConditional mice specific for PGC-1αwere introduced from the Jackson Laboratory, USA and initially inbred to obtain homozygote PGC-1αmice. The PGC-1αconditional mice were further crossed with Dlx5/6-Cre-IRES-EGFP transgenic mice to achieve specific knockout of PGC-1α in the GABAergic interneuron.
RESULTSThe offspring with specific knockout PGC-1α gene were successful for the generation of GABAergic interneuron, with the resulting genotype being PGC-1α.
CONCLUSIONThe PGC-1αmice were obtained through a proper crossing strategy, which has provided a suitable platform for studying the function of PGC-1α in neuropsychiatric diseases.
