Identification of a novel HLA allele HLA-B*46:01:18.
- Author:
Yongfeng PEI
1
;
Huini HUANG
;
Hengcong LI
;
Weidong SHEN
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Alleles; Asian Continental Ancestry Group; genetics; Base Sequence; China; Exons; Female; HLA-B Antigens; genetics; Humans; Male; Molecular Sequence Data; Young Adult
- From: Chinese Journal of Medical Genetics 2017;34(2):247-250
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo report on a novel human leukocyte antigen (HLA) allele.
METHODSPolymerase chain reaction-sequence based typing was used for routine HLA typing. For one sample, the result of B locus typing showed mismatch of one base with B*46:01:01, B*15:25:01 at locus 384. The group specific sequencing primers, which target at B*46 and B*15, were used to confirm the difference between the novel allele and the highest homologous allele.
RESULTSThe sequencing results showed that the highest homologous allele to the novel allele was B*46:01:01. The two sequences only differed for position 384 within the exon 3 (384G>T), which resulted in a codon change (GGG>GGT), though the amino acid sequence of the novel allele at position 104 was still Glycine (G). Investigation of the family showed that the novel allele was inherited from the father.
CONCLUSIONThe novel HLA-B allele, discovered in ethnic Zhuangs from Guangxi, has been designated as HLA-B *46:01:18 by the World Health Organization (WHO) HLA Nomenclature Committee.
