Analysis of genetic polymorphisms and mutations of 20 frequently used STR loci among ethnic Hans from Henan.
- VernacularTitle:河南汉族人群20个常用STR基因座的遗传多态性及突变研究
- Author:
Hongdan WANG
1
;
Bing KANG
;
Yue GAO
;
Xiaodong HUO
;
Tao LI
;
Qiannan GUO
;
Bofeng ZHU
;
Shixiu LIAO
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Alleles; Asian Continental Ancestry Group; ethnology; genetics; Child; Child, Preschool; China; ethnology; Female; Genotype; Humans; Male; Microsatellite Repeats; Mutation; Pedigree; Polymorphism, Genetic; Young Adult
- From: Chinese Journal of Medical Genetics 2017;34(2):266-269
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the genetic polymorphisms and mutations of 20 frequently used autosomal microsatellites among ethnic Hans from Henan.
METHODSPeripheral blood samples of 2604 individuals were collected. DNA was amplified and genotyped using a PowerPlex(TM) 21 system. The frequencies, forensic parameters and mutation rates of the 20 short tandem repeat (STR) loci were analyzed.
RESULTSA total of 323 alleles were found in this population and the allelic frequencies have ranged from 0.0003 to 0.5144. Except for D3S1358, TH01 and TPOX, mutations have been found in all of the remaining 17 STR loci, which totaled 47, with mutation rates ranging from 0 to 3.46 × 10.
CONCLUSIONThe 20 STR loci selected by the PowerPlex(TM) 21 system are highly polymorphic among ethnic Hans from Henan, and may be of great value in forensic and human population studies. As no similar study has been carried out previously, above results may be of great value for individual discrimination and paternal testing.
