Allelic diversity of KIR2DL4 gene and identification of five novel alleles among southern Han Chinese population.
- VernacularTitle:南方汉族人群KIR2DL4基因的多态性及五个新等位基因的鉴定
- Author:
Guobin ZHANG
1
,
2
;
Zhihui DENG
Author Information
- Publication Type:Journal Article
- MeSH: Alleles; Asian Continental Ancestry Group; ethnology; genetics; Base Sequence; China; ethnology; Gene Frequency; Genetic Variation; Genotype; Haplotypes; Humans; Receptors, KIR2DL4; genetics
- From: Chinese Journal of Medical Genetics 2017;34(2):270-274
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the polymorphisms of the KIR2DL4 gene in a southern Han Chinese population.
METHODSGenomic DNA isolated from 306 unrelated individuals was amplified by using KIR2DL4-specific PCR primers. The PCR products were genotyped for the entire coding sequence by sequencing-based typing (SBT). Assignment of allelic profile was accomplished with an Assign 3.5 software. For samples with inconclusive SBT results, the RT-PCR products covering the entire coding sequence of the KIR2DL4 gene were subjected to cloning and haplotype sequencing.
RESULTSAmong the 306 individuals, 11 alleles were detected, of which 5 novel alleles were officially named by the KIR subcommittee of the World Health Organization Nomenclature Committee for factors of HLA system. The observed frequencies for the 11 alleles were KIR2DL4 *00102 (75.5%), *00103 (8.2%), *00501 (34.0%), *00503 (0.7%), *00504 (0.7%), *00602 (14.4%), *00801 (11.4%), *011 (22.2%), *032 (0.3%), *033 (0.3%) and *034 (0.3%). The ratio of 10A type alleles including 2DL4*00102, *00103, *00501, *00503, *00504, *00602, *032, *033, *034 and 9A type alleles including 2DL4*00801, *011 were 97.0% and 33.0%, respectively, with a ratio of 2.9:1.
CONCLUSIONThe allelic diversity of the KIR2DL4 gene in southern Han Chinese has been elucidated, which may provide valuable data for research on transplantation, reproductive immunity, KIR-associated disease and evolution.
