Evaluation of anti-apoptotic activity of different dietary antioxidants in renal cell carcinoma against hydrogen peroxide.
- Author:
Neeraj K GARG
1
;
Sharad MANGAL
;
Tejram SAHU
;
Abhinav MEHTA
;
Suresh P VYAS
;
Rajeev K TYAGI
Author Information
- Publication Type:Journal Article
- Keywords: Anticancer; Antioxidants; Apoptosis; Hydrogen peroxide; RCC-26; ROS
- MeSH: Antioxidants; pharmacology; Apoptosis; drug effects; Carcinoma, Renal Cell; Cell Line, Tumor; Cell Survival; drug effects; DNA Fragmentation; drug effects; Humans; Hydrogen Peroxide; toxicity; Oxidative Stress; drug effects; Reactive Oxygen Species; analysis; metabolism; Vitamins; pharmacology
- From:Asian Pacific Journal of Tropical Biomedicine 2011;1(1):57-63
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo evaluate the anti-apoptotic and radical scavenging activities of dietary phenolics, namely ascorbic acid,α-tocopherol acetate, citric acid, salicylic acid, and estimate H2O2-induced apoptosis in renal cell carcinoma cells.
METHODSThe intracellular antioxidant potency of antioxidants was investigated. H2O2-induced apoptosis in RCC-26 was assayed with the following parameters: cell viability (% apoptosis), nucleosomal damage and DNA fragmentation, bcl-2 levels and flow cytometery analysis (ROS production evaluation).
RESULTSThe anticancer properties of antioxidants such as ascorbic acid, α-tocopherol acetate, citric acid, salicylic acid with perdurable responses were investigated. It was observed that these antioxidants had protective effect (anti-apoptotic activity) against hydrogen peroxide (H2O2) in renal cell carcinoma (RCC-26) cell line.
CONCLUSIONSThis study reveals and proves the anticancer properties. However, in cancer cell lines anti-apoptotic activity can indirectly reflect the cancer promoter activity through radicals scavenging, and significantly protect nucleus and bcl-2.
