Relationship of collagen type I alpha 1 and alpha 2 gene polymorphisms with bone mineral density.
- Author:
Dong-feng LI
1
;
Wen WU
;
Xue-zhen CAI
;
Yan-hong YANG
;
Kai LIN
;
Xiao-sui HUANG
;
Ting DONG
;
Xi-mei ZHI
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Bone Density; China; Collagen; genetics; Collagen Type I; genetics; Female; Gene Frequency; Genotype; Humans; Lumbar Vertebrae; metabolism; Male; Middle Aged; Polymorphism, Genetic; Polymorphism, Restriction Fragment Length
- From: Journal of Southern Medical University 2006;26(7):981-990
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate collagen type I alpha 1 (COL1A1) and alpha 2 (COL1A2) gene polymorphisms in Chinese and their relationship with bone mineral density.
METHODSTotalling 628 residents of Han nationality in Guangzhou aged 53.4-15.9 (range 20-79) years were surveyed for COL1A1 and COL1A2 gene genotypes by polymerase chain reaction-restriction fragment length polymorphism. Bone mineral density of the lumbar vertebrae, greater trochanter, femur neck and Ward's triangle was measured by dual-energy X-ray absorptiometry.
RESULTSCOL1A1 Sp1 polymorphism was not found in these subjects, and the genotype of all samples were type SS. COL1A2 genotyping revealed the distribution of EE genotype in 49.7%, Ee in 40.9% and ee in 9.4% of the subjects. The frequency distribution of EcoR1 alleles followed the Hardy-Weinberg equilibrium. The mean bone mineral density did no significantly differ among these genotype groups (P>0.05 by analysis of variance).
CONCLUSIONCOL1A1 Sp1 binding site polymorphism is absent and COL1A2 EcoR1 site polymorphism is not associated with bone mineral density in Chinese of Han nationality.
