Exercises at different intensities and sequential changes of rat skeletal muscle cell apoptosis.
- Author:
Bao-ta CAI
1
;
Bin YU
;
Jiang-ping ZHAO
;
Xing ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; physiology; Flow Cytometry; Male; Muscle, Skeletal; cytology; physiopathology; Physical Conditioning, Animal; physiology; Random Allocation; Rats; Rats, Sprague-Dawley; Time Factors
- From: Journal of Southern Medical University 2006;26(7):1017-1019
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of exercises of different intensities on rat skeletal muscle cell apoptosis.
METHODSRat models of exhaustive exercise-induced skeletal muscle injury was established using tread mill exercises at different speeds, and the skeletal muscle cell apoptosis was detected using propidium iodide (PI) staining and flow cytometry.
RESULTNo obvious gastrocnemius cell apoptosis was observed in rats with normal exercise (P>0.05), but the cell apoptosis index was statistically significant in moderate and exhaustive exercise groups (P<0.05), reaching the highest level in exhaustive exercise group after exercising. The gastrocnemius cell apoptosis index increased obviously on days 1 and 3 of exercise, and stabilized in moderate and exhaustive exercise groups.
CONCLUSIONExercises can evoke cell apoptosis and accelerate apoptotic cell clearance, and the imbalance between the two events during exercises may contribute to skeletal muscle injury.
