Therapeutic effect of glucose-6-phosphate polyclonal antibody on vasogenic brain edema in rats.
- Author:
Zhen TAO
1
;
Bing-xun LU
;
Yun-zhou ZHANG
;
Gang CHENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies, Monoclonal; immunology; therapeutic use; Blood-Brain Barrier; drug effects; physiopathology; Brain Edema; chemically induced; drug therapy; physiopathology; Capillary Permeability; drug effects; Female; Glucose-6-Phosphate; immunology; Male; Phenylephrine; Random Allocation; Rats; Rats, Wistar
- From: Journal of Southern Medical University 2006;26(7):1041-1043
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the therapeutic effect of glucose-6-phosphate polyclonal antibody (G-6-P pAb) on vasogenic brain edema (VBE) in rats.
METHODSSixty Wistar rats were randomly divided into normal control group, VBE group, mannitol-treated edema group, and G-6-P pAb-treated edema group. After establishment of rat models of VBE by intraperitoneal injection of phenylephrine in the latter 3 groups, mannitol was injected through the femoral vein in mannitol group and G-6-P pAb injected intraperitoneally in G-6-P pAb group. The permeability of the blood-brain barrier (BBB) was determined by Evans blue (EB) extravasation method, and the brain water content in the gray and white matter measured with a moisture analyzer.
RESULTSG-6-P pAb administration significantly reduced the permeability of BBB as well as the water content in the white matter in comparison with mannitol treatment (P<0.01), but the two treatments showed no obvious difference in reducing the water content in the gray matter (P>0.05).
CONCLUSIONChanges in G-6-P activity results in BBB permeability alteration in the condition of VBE, and G-6-P pAb has a selective therapeutic effect against VBE, especially white matter edema.
