Differentially express genes in human embryonic lung fibroblasts with damage tolerance induced by low-dose hydroquinone.
- Author:
Qin-zhi WEI
1
;
Zhi-xiong ZHUANG
Author Information
- Publication Type:Journal Article
- MeSH: Adaptation, Physiological; drug effects; Cells, Cultured; Dose-Response Relationship, Drug; Fibroblasts; cytology; drug effects; metabolism; Gene Expression; drug effects; Gene Expression Profiling; Humans; Hydroquinones; pharmacology; Lung; cytology; embryology; metabolism; Polymerase Chain Reaction; methods
- From: Journal of Southern Medical University 2006;26(8):1092-1095
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the differentially expressed genes in human embryonic lung fibroblasts (HELF) induced by small-dose hydroquinone (HQ) using fluorescence differential display-PCR (DD-PCR).
METHODSAccording to the dose-effect relation of HQ toxicity we established previously, HQ dose that did not induce observed cell damage or proliferation arrest was defined as low dose (100 pmol/L), and that causing obvious cell damage as the high dose (100 micrommol/L). The cells were then treated with low or high dose of HQ, or exposed to high-dose HQ following pretreatment with low-dose HQ for some time, respectively. Fluorescence DD-PCR was performed and 33 differentially expressed genes were identified in the cells with different treatments, and 8 of the identified genes were amplified, cloned, sequenced and blasted.
RESULTSSeven of the 8 amplified genes were unknown genes, and the left one was identified as a known gene highly homologous to that encoding Homo sapiens Rap1 interacting factor 1 (RIF1).
CONCLUSIONLow-dose HQ can induce damage tolerance in HELF, and identification of the differentially expressed genes may provide valuable sight into the mechanism of HQ-induced damage tolerance.
