Effects of Saponin B from Anemarrhena asphodeloides Bunge on tau hyperphosphorylation induced by beta-amyloid peptide (25-35) in rats.
- Author:
Lei ZHONG
1
;
Jie TAN
;
Shi OUYANG
;
Jiang-ping XU
Author Information
- Publication Type:Journal Article
- MeSH: Amyloid beta-Peptides; pharmacology; Anemarrhena; chemistry; Animals; Hippocampus; drug effects; metabolism; Immunohistochemistry; Intercellular Signaling Peptides and Proteins; biosynthesis; genetics; Male; Peptide Fragments; biosynthesis; genetics; pharmacology; Phosphorylation; drug effects; RNA, Messenger; biosynthesis; genetics; Random Allocation; Rats; Rats, Sprague-Dawley; Reverse Transcriptase Polymerase Chain Reaction; Saponins; pharmacology; Tumor Suppressor Protein p53; biosynthesis; genetics; tau Proteins; metabolism
- From: Journal of Southern Medical University 2006;26(8):1106-1109
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the changes in the expressions of p53, DKK (the inhibitor of Wnt pathway) and phosphorylated tau in rat bilateral hippocampus after beta-amyloid peptide (beta-AP) (25-35) injection, and observe the effect of saponin B from Anemarrhena asphodeloides Bunge (SAaB) in this model.
METHODSAfter bilateral injection of beta-AP (25-35) into the hippocampus of rats, RT-PCR was performed for observing the changes in p53 and DKK mRNA expressions and immunochemistry carried out to detect the changes in phosphorylated tau protein.
RESULTSRT-PCR showed increased p53 and DKK mRNA expression and immunochemistry revealed increased phosphorylated tau-positive cells in rat hippocampus after beta-AP (25-35) injection, and administration of SAaB significantly ameliorated these changes.
CONCLUSIONSAaB can significantly ameliorate beta-AP-induced tau hyperphosphorylation by inhibiting increased p53 and DKK mRNA expressions in response to beta-AP injection.
