Labeling of CDTPA-dianhydride-coupled CD45 monoclonal antibody with yttrium-90.
- Author:
Yun-bi FU
1
;
Gui-ping LI
;
Fan-yi MENG
Author Information
- Publication Type:Journal Article
- MeSH: Anhydrides; chemistry; Antibodies, Monoclonal; chemistry; immunology; Humans; Immunoconjugates; chemistry; immunology; Isotope Labeling; methods; Leukocyte Common Antigens; immunology; Pentetic Acid; chemistry; Radiopharmaceuticals; chemical synthesis; chemistry; immunology; Yttrium Radioisotopes; chemistry
- From: Journal of Southern Medical University 2006;26(8):1230-1232
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the methods for labeling CDTPA-coupled CD45 monoclonal antibody (mAb) with yttrium-90 ((90)Y) for potential acute myeloid therapy.
METHODSCD45 mAb was labeled with (90)Y by CDTPA and the labeling rate, radiochemical purity, final specific activity, and immunological activity of the mAb were detected.
RESULTSWith the optimal molar ratio of CDTPA/Ab at 20:1, the labeling rate was 95%, radiochemical purity 99.8%, and final specific activity 1.9 mCi/mg. This conjugate was stable in vitro with comparable immunological activity in comparison with unlabeled CD45 mAb.
CONCLUSION(90)Y-CDTPA-CD45 mAb possesses good properties as an ideal targetting therapeutic agent for acute leukemia.
