Effect of transforming growth factor alpha on the expression of cyclin E and cyclin D1 in gastric carcinoma cells.
- Author:
Wei-jiang LIANG
1
;
Wan-dai ZHANG
;
Cun-long CHEN
;
Yong-li YAO
;
Rong-cheng LUO
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; metabolism; pathology; Cell Cycle; drug effects; Cell Line, Tumor; Cyclin D1; biosynthesis; Cyclin E; biosynthesis; Flow Cytometry; Fluorescent Antibody Technique; Humans; Stomach Neoplasms; metabolism; pathology; Transforming Growth Factor alpha; pharmacology
- From: Journal of Southern Medical University 2006;26(8):1255-1257
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of transforming growth factor alpha (TGFalpha) on the expression of cyclin E and D1 in gastric carcinoma cells.
METHODSHuman gastric adenocarcinoma SGC7901 cells were cultured routinely and synchronized at G(0)/G(1) phase in serum-free RPMI-1640. The percentage of the cells at G(0)/G(1) phase was detected by propidium iodide staining and flow cytometry (FCM), and the synchronized cells were cultured in RPMI-1640 supplemented with 2.5% calf serum and treated with 10, 30, and 50 microg/L TGFalpha for 5 h. The expression of cyclin E and D1 in SGC7901 cells was detected by immunofluorescent staining and FCM.
RESULTSThe percentage of the cells at G(0)/G(1) phase increased from 54% in routine culture to 72% in the serum-free RPMI-1640 culture. TGFalpha treatment of the cells synchronized at G(0)/G(1) phase induced significant increment of cyclin E and D1 expressions (P<0.001), and at the dose of TGFalpha of 50 microg/L, their expressions increased by 25.18% and 27.52%, respectively (P<0.001).
CONCLUSIONTGFalpha can increase the expression of cyclin E and D1 in gastric carcinoma cells to promote their cell cycle progress.
