Effects of prenatal stress on kinetic properties of high-voltage-activated Ca2+ channel in freshly isolated offspring rat hippocampal CA3 pyramidal neurons.
- Author:
Qing CAI
1
;
Zhong-liang ZHU
;
Xiao-li FAN
;
Ning JIA
;
Zhuan-li BAI
;
Xia LI
;
Liang SONG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Calcium Channels; physiology; Female; Hippocampus; cytology; Kinetics; Male; Membrane Potentials; physiology; Patch-Clamp Techniques; Pregnancy; Pregnancy Complications; psychology; Pyramidal Cells; cytology; physiology; Rats; Rats, Sprague-Dawley; Restraint, Physical; psychology; Stress, Psychological; physiopathology
- From: Journal of Southern Medical University 2006;26(9):1288-1292
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of prenatal stress (PNS) on kinetic properties of high-voltage-activated (HVA) Ca(2+) channel in freshly isolated offspring rat hippocampal CA3 pyramidal neurons.
METHODSThe pregnant rats were exposed to restraint stress, and the hippocampal CA3 pyramidal neurons were freshly isolated from the offspring rats to record voltage-gate Ca(2+) channel currents in these neurons using whole-cell configuration of patch-clamp technique.
RESULTSPNS significantly increased HVA calcium current density and integral current but did not alter the steady-state activation and steady-state inactivation properties of HVA Ca(2+) channel in the offspring rat CA3 neurons. The maximal HVA calcium current density was -40.89-/+0.31 pA/pF in the control group (n=10) and -49.44-/+0.37 pA/pF in PNS group (n=8, P<0.01). The maximal integral current of the HVA Ca(2+) channel was 106.81-/+4.20 nA*ms in the control group (n=10) and 133.49-/+2.59 nA*ms in the PNS group (n=8, P<0.01).
CONCLUSIONMaternal exposure to stress during the critical phase of pregnancy may result in long-lasting effects on the ion channels of the hippocampal neurons in the offspring rats.
