Fenofibrate inhibits tumor necrosis factor-α-induced expression of CD40 and matrix metalloproteinase in human vascular endothelial cells
10.3321/j.issn:1673-4254.2006.10.001
- VernacularTitle:非诺贝特对TNF-α诱导的人脐静脉内皮细胞CD40表达和基质金属蛋白酶活性的抑制作用
- Author:
Rong LIN
1
;
Jun-Tian LIU
;
Wei-Jie GAN
;
Wei-Rong WANG
;
Chun-Jie HAN
;
Yu LIU
;
Zhi-Yuan FANG
Author Information
1. Department of Pharmacology
- Keywords:
fenofibrate;
CD40;
matrix metalloproteinase-2;
matrix metalloproteinase-9
- From:
Journal of Southern Medical University
2006;26(10):1383-1387
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the regulatory effects of fenofibrate on TNF-α-induced CD40 expression and matrix metalloproteinase (MMP) activity in human vascular endothelial cells (HUVECs). Methods Quantitative RT-PCR and flow cytometry were employed to evaluate the effect of fenofibrate on TNF-α-induced CD40 mRNA and cell surface CD40 expression in HUVECs, and gelatin zymography was used to determine the effect of fenofibrate on the gelatinolytic activities of MMP-2 and MMP-9 in TNF-α-stimulated HUVECs. Results Fenofibrate at the concentrations of5×10-5, 1×10-4 and 2×10-4mol/L significantly reduced TNF-α-induced increment of CD40 mRNA and cell surface CD40 expressions (P＜0.01), with the maximal inhibition achieved at the concentration of 1 × 10-4 mol/L. Fenofibrate at 2× 10-4 mol/L did not further decrease CD40expression induced by TNF-α. Fenofibrate significantly inhibited the stimulatory effect of TNF-α on MMP-2 and MMP-9 activities in HUVECs. Conclusion Fenofibrate reduces TNF-α-induced increment of CD40 expression and MMP-2 and MMP-9 activities in HUVECs.
