Subcellular localization of APOBEC3G by confocal laser scanning microscope (CLSM).
- Author:
Yi-Shu YANG
1
;
Lan LI
;
Ze-Lin LI
;
Yi ZENG
Author Information
1. College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100022, China. yishu-y@bjut.edu.cn
- Publication Type:Journal Article
- MeSH:
APOBEC-3G Deaminase;
Cell Line;
Cytidine Deaminase;
Cytoplasm;
metabolism;
DNA, Complementary;
genetics;
isolation & purification;
metabolism;
Green Fluorescent Proteins;
genetics;
metabolism;
HeLa Cells;
Humans;
Microscopy, Confocal;
methods;
Nucleoside Deaminases;
genetics;
metabolism;
Recombinant Fusion Proteins;
genetics;
metabolism;
Repressor Proteins;
genetics;
metabolism;
Reverse Transcriptase Polymerase Chain Reaction
- From:
Chinese Journal of Virology
2007;23(1):16-21
- CountryChina
- Language:Chinese
-
Abstract:
Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3G (APOBEC3G) cDNA was amplified from total RNA prepared from nonpermissive H9 cells by RT-PCR. APOBEC3G cDNA is 1155nt long, encoding 384 amino acids. The APOBEC3G gene was then cloned into the eukaryotic expression vector pEGFP-C3. The generated pEGFP-3G construct was then transfected into CD4+ HeLa cell to determine the expression and the subcellular localization of GFP-APOBEC3G fusion protein. Under CLSM the localization of the expressed GFP-APOBEC3G in the cytoplasm of CD4+ HeLa cells was observed.
