Silencing HSV1 gD expression in cultured cells by RNA interference.
- Author:
Qin-Chang ZHU
1
;
Zhe REN
;
Chun-Long ZHANG
;
Mei-Ying ZHANG
;
Hong-Juan LIAO
;
Qiu-Ying LIU
;
Pei-Zhuo ZHANG
;
Jiu-Xiang LI
;
Chao-Feng HU
;
Hua-Dong WANG
;
Yi-Fei WANG
Author Information
1. Biomedicine Research and Development Center of Jinan University, Guangzhou 510632, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Cercopithecus aethiops;
Genetic Vectors;
genetics;
Green Fluorescent Proteins;
genetics;
metabolism;
Herpesvirus 1, Human;
genetics;
Humans;
Polymerase Chain Reaction;
RNA Interference;
RNA, Small Interfering;
genetics;
Recombinant Fusion Proteins;
genetics;
metabolism;
Transfection;
Vero Cells;
Viral Envelope Proteins;
genetics;
metabolism
- From:
Chinese Journal of Virology
2007;23(1):22-27
- CountryChina
- Language:Chinese
-
Abstract:
To explore the anti-HSV-1 effect of silencing gD gene expression by RNA interference, five 21-nucleotide duplex small interfering RNAs(siRNAs) targeting the HSV1 gD sequence were designed and the gD-EGFP fusion gene expression vector was constructed, then co-transfected into Vero cell, and screened the effective siRNA through analyzing the intensity of the EGFP fluorescence. Finally, the anti-HSV1 effect was confirmed by plaque reduction assay, real-time PCR and daughter virus titration of HSV1 infected Vero cells transfected with siRNAs. The study demonstrated that siRNAs could effectively and specifically inhibit gD gene expression in HSV1-infected cells, but only had a little effect on HSV1 infection, so taking gD as the target of siRNA against HSV1 needs further study.
