Sequence analysis of norovirus CHN02/LZ35666 from Lanzhou city of China based on RdRp and VP1 genes.
- Author:
Miao JIN
1
;
Jing-Feng FAN
;
Tian-Fei YU
;
Zhao-Yin FANG
;
Jun-Wei WANG
Author Information
1. Department of Preventive Veterinary, College of Veterinary Medicine, Northeast Agriculture University, Harbin 150030, China. fcsjinmiao1321@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Amino Acid Sequence;
Base Sequence;
Capsid Proteins;
genetics;
China;
Gastroenteritis;
virology;
Humans;
Molecular Sequence Data;
Norovirus;
classification;
genetics;
Phylogeny;
RNA Replicase;
genetics;
Sequence Alignment;
Sequence Analysis, DNA
- From:
Chinese Journal of Virology
2007;23(1):63-67
- CountryChina
- Language:Chinese
-
Abstract:
Sequence analysis of a new norovirus (NV) isolated from Lanzou city of China was performed based on partial sequence of RNA dependent RNA polymerase (RdRp) and complete capsid protein (VP1) gene. The isolated strain CHN02/LZ35666 shared high sequence homology with GII-4 NVs. Nucleotide homologies of RdRp region and encoded capsid protein region were 90.4% -- 98.6% and 89.8% -- 95.7% , respectively, while amino acid homology of capsid protein region was 94.4% -- 97.4%. The analysis of GDD motif in RdRp region indicated this GDD motif of Lanzhou strain differed from those of the GII-4 predominant epidemic strains. Lanzhou strain formed an independent branch in GII-4 cluster in the phylogenetic tree based on nucleotide sequence of RdRp region and amino acid sequence of capsid protein. Sequence alignment revealed a mutation at the fourth key site of the receptor-binding interface in the strains isolated after 2002 compared with those of previous strains suggesting a possible change of binding pattern to HBGAs receptors.
