Molecular analysis on the capsid gene and 3' untranslation region of three Getah viruses isolated in China.
- Author:
You-gang ZHAI
1
;
Huan-qing WANG
;
Shi-hong FU
;
Guo-dong LIANG
Author Information
1. National Institute for Viral Disease Control and Prevention, China CDC, Beijing 100052, China.
- Publication Type:Journal Article
- MeSH:
3' Untranslated Regions;
genetics;
Alphavirus;
classification;
genetics;
isolation & purification;
Base Sequence;
Capsid Proteins;
genetics;
China;
Molecular Sequence Data;
Phylogeny;
Sequence Homology, Nucleic Acid
- From:
Chinese Journal of Virology
2007;23(4):270-275
- CountryChina
- Language:Chinese
-
Abstract:
To compare the molecular characteristics of the Capsid gene and 3' untranslation region (3'UTR) of Getah viruses (GETV) isolated in Hainan Province and Hebei Province of China,the viral RNAs were extracted from M1(Hainan), HB0215-3 and HB0234(Hebei) virus stocks. Capsid gene segments and 3' UTR segments from three strains of Chinese GETV were obtained by RT-PCR and then sequenced. The obtained nucleotide sequences were analyzed using the Clustal X(1.8), DNASTAR, MAGA3.1 programs. The full-length Capsid gene of the 3 strains of Chinese GETV were comprised of 801, 804 and 804 nucleotides each, encoding the protein of 267,268 and 268 amino acids each. Sequencing of Capsid gene fragments showed that two strains of Hebei isolates were identical and had homology of 97.6% at nucleotide level and 97.8% at amino acids level with M1. Their homologies when compared with strains isolated from other countries were also high at nucleotide levels (95.4%-99.6%). The 3'UTR from the three strains were comprised of 411, 401 and 401 nucleotides each, and had found specific deletion of 10 nt at position 44-54 and two specific nucleotide sites that was T at position 64 and C at position 148. GETV isolated in China presented relation of the year of virus isolation with the phylogenesis distance when compared with the other GETV strains and comprised a genetically highly conserved group.
