Identification of a new Puumala hantavirus subtype in rodents from China.
- Author:
Li-hua TANG
1
;
Quan-fu ZHANG
;
Mei-hong XIU
;
Guang-wei GU
;
Shen BO
;
Xian-da YANG
;
Mi-fang LIANG
;
De-xin LI
Author Information
1. State Key Laboratory for Infectious Disease Control and Prevention, National Institute for Viral Diseases Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China. tlhfree@hotmail.com
- Publication Type:Journal Article
- MeSH:
Animals;
Base Sequence;
Cercopithecus aethiops;
China;
Evolution, Molecular;
Genome, Viral;
Hantavirus;
genetics;
Molecular Sequence Data;
Phylogeny;
Puumala virus;
classification;
genetics;
isolation & purification;
Reverse Transcriptase Polymerase Chain Reaction;
Rodentia;
virology;
Sequence Analysis, DNA;
Vero Cells
- From:
Chinese Journal of Virology
2007;23(4):320-325
- CountryChina
- Language:Chinese
-
Abstract:
In order to detect the nucleic acid of Puumala hantavirus, RNA was extracted from lungs of bank voles captured in Northeast China, and partial S and M genome segments of Puumala virus were amplified by RT-PCR and sequenced. Phylogenetic analysis suggested that Chinese Puumala virus had diverged from the common node of PUUV, with accumulating nucleotide substitutions and formed a distinct lineage from other Puumala viruses. Newly found Puumala virus was most closely related to the Kamiiso-8Cr-95 and Tobetsu-60Cr-93 strains which came from Japan and the muju strains which came from South Korea. By analysis of S and M genome segments of Puumala virus, we deduced a new Puumala virus subtype did exist in Northeast China.
