Cloning NS1 gene of H5N1 avian influenza virus and apoptosis induced by it in human pulmonary carcinoma cell line A549.
- Author:
Chuan-Fu ZHANG
1
;
Shi-Wei JIANG
;
Heng-Qi ZHU
;
Yu-Tao YANG
;
Zhi-Xin YANG
;
Long XU
;
Li-Xia ZHAO
;
Xiao-Wei ZHOU
;
Pei-Tang HUANG
Author Information
1. Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China. hnzcf@126.com
- Publication Type:Journal Article
- MeSH:
Annexin A5;
analysis;
Apoptosis;
Cell Line, Tumor;
Cloning, Molecular;
Humans;
Influenza A Virus, H5N1 Subtype;
genetics;
pathogenicity;
Lung Neoplasms;
pathology;
Viral Nonstructural Proteins;
genetics;
physiology
- From:
Chinese Journal of Virology
2007;23(5):360-365
- CountryChina
- Language:Chinese
-
Abstract:
The NS1 gene of the H5N1 subtype avian influenza virus was amplified by RT-PCR, and the am-plified product was cloned into the eukaryotic expression vector pCMV-Myc, then it was transfected into A549 cells. After 48 h, the expression of NS1 was detected by Western blot. Fluorescence and electron microscopy and flow cytometry showed that the NS1 gene of H5N1 avian influenza virus could induce apop-tosis in human pulmonary carcinoma cell line A549.
