Construction and immunogenicity study of DNA vaccine expressing human H5N1 influenza virus hemagglutinin.
- Author:
Li-Fang HUAN
1
;
Li-Hong YAO
;
Ai-Jun CHEN
;
Cong-Sheng CHENG
;
Run-Qing JIA
;
Qing TIAN
;
Hong BO
;
Jian-Qiang GUO
;
Min WANG
;
Yue-Long SHU
;
Zhi-Qing ZHANG
Author Information
1. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China. lifanghuan99@hotmail.com
- Publication Type:Journal Article
- MeSH:
Animals;
Antibodies, Viral;
blood;
COS Cells;
Cercopithecus aethiops;
Female;
Hemagglutinin Glycoproteins, Influenza Virus;
genetics;
immunology;
Immunoglobulin G;
blood;
Influenza A Virus, H5N1 Subtype;
immunology;
Influenza Vaccines;
immunology;
Mice;
Mice, Inbred BALB C;
Vaccines, DNA;
immunology
- From:
Chinese Journal of Virology
2007;23(5):366-370
- CountryChina
- Language:Chinese
-
Abstract:
Based on the first isolated human H5N1 influenza virus strain A/Anhui/1/2005 in China, HA and HA1 genes were amplified and cloned into the eukaryotic expression vector pStar. The recombinant plasmids pStar-HA and pStar-HA1 were transfected into COS7 cells. Western blot and IFA showed that the two recombinant DNA plasmids were successfully expressed in eukaryotic cells. BALB/c mice were immunized with the plasmids DNA by intramuscular injection. Anti-HA specific antibody in peripheral blood of immunized mice was tested by ELISA. The results showed that the recombinant plasmids successfully induced the anti-HA humoral immune response, and there was no significant difference between HA and HA1 as immunogen. This work provides basis for future development of novel avian flu vaccine.
