Genome sequencing and analysis of foot-and-mouth disease virus Asia1/YNBS/58 strain.
- Author:
Hui-Yun CHANG
1
;
Jun-Zheng DU
;
Guo-Zheng CONG
;
Jun-Jun SHAO
;
Tong LIN
;
Qing-Ge XIE
Author Information
1. Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China. changhuiyun@126.com
- Publication Type:Journal Article
- MeSH:
3' Untranslated Regions;
chemistry;
Capsid Proteins;
genetics;
Foot-and-Mouth Disease Virus;
genetics;
Genome, Viral;
Phylogeny;
Sequence Analysis, DNA
- From:
Chinese Journal of Virology
2007;23(5):407-411
- CountryChina
- Language:Chinese
-
Abstract:
The full-length genomic sequence of foot-and-mouth disease virus (FMDV) Asia1/YNBS/58 strain was determined by RT-PCR and compared with other 17 reference strains. The results showed that the complete genome of Asia1/YNBS/58 was 8164nt long including a 1061-nt 5' untranslated region (UTR), a 6990-nt open reading frame (ORF), and a 113-nt 3'UTR. The homology analysis indicated that the UTR regions and non-structural proteins were more conserved than the structural proteins in FMDV. VP1 exhibited the lowest conservation and VP4 was exceptionally conserved. The VP1-, VP2-, and VP3-based phylogenetic trees were divided into distinct clusters according to different serotypes, while the other gene-based phylogenetic trees exhibited some degree of intercross among serotypes. This study is the first description of the full-length genomic sequence of FMDV Chinese serotype Asia1.
