Laboratory diagnosis and molecular characterization of highly pathogenic avian influenza virus (H5N1) in human in Hunan Province in 2005-2006.
- Author:
Yi-Wei HUANG
1
;
Zi LI
;
Hong ZHANG
;
Yun-Zhi LIU
;
Le-Ying WEN
;
Jun-Hua LI
;
Li-Dong GAO
;
Xin-Sheng ZHAO
;
Zhong-Jie LI
;
Bi-Yun CHEN
;
Yu LAN
;
Lei ZHOU
;
Yue-Long SHU
Author Information
1. Hunan Provincial Center for Disease Control and Prevention, Changsha 410005, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Antibodies, Viral;
blood;
Child;
China;
Female;
Hemagglutination Inhibition Tests;
Hemagglutinin Glycoproteins, Influenza Virus;
genetics;
Humans;
Influenza A Virus, H5N1 Subtype;
classification;
genetics;
immunology;
isolation & purification;
Influenza, Human;
diagnosis;
virology;
Male;
Phylogeny
- From:
Chinese Journal of Virology
2007;23(6):434-439
- CountryChina
- Language:Chinese
-
Abstract:
To determine the etiologic agents of two atypical pneumonia human cases in Hunan Province in 2005-2006 and to study their pathogenic potential, the patients' respiratory tract samples and sera were collected. The respiratory tract samples were tested by real-time RT-PCR and RT-PCR methods, and the sera by hemagglutination-inhibition assay. Virus was isolated from case 2 and its genome was sequenced and analyzed. Results showed the H5 nucleic acid tests of two cases were positive. The H5-specific antibody titer of the convalescence serum of case 1 showed a 4-fold greater rise than that of the acute phase. And case 2's antibody titer of acute phase was negative. The two atypical pneumonia cases were confirmed as the avian influenza A (H5N1) infection cases. Viral strain A/Hunan/1/2006 was isolated from case 2. Phylogenetic and molecular analysis suggested that 8 gene segments of A/Hunan/1/2006 originated from avian viruses. And A/Hunan/1/2006 was similar with viruses isolated from avian in Hunan Province. The isolated virus did not recombine with human influenza viruses and no obvious variation was observed.
