The molecular differences between genotype 1 and genotype 4 of HEV in their neutralization region.
- Author:
Qing-Shun GUO
1
;
Sheng-Xiang GE
;
Jun-Hui XIONG
;
Qiang YAN
;
Shao-Wei LI
;
Ying GU
;
Ping-Dong XU
;
Wei-Guo SHI
;
Jun ZHANG
;
Ning-Shao XIA
Author Information
1. National Institute of Diagnostics and Vaccine Development in Infectious Diseases, Xiamen University, Xiamen 361005, China).
- Publication Type:Journal Article
- MeSH:
Antibodies, Monoclonal;
immunology;
Base Sequence;
Genotype;
Hepatitis E virus;
classification;
genetics;
immunology;
Humans;
Immunodominant Epitopes;
Molecular Sequence Data;
Mutation;
Neutralization Tests;
Open Reading Frames
- From:
Chinese Journal of Virology
2007;23(6):454-458
- CountryChina
- Language:Chinese
-
Abstract:
HEV is classified into H (human) group and Z (zoonosis) group according to its compatible host. H group contains genotype 1 and genotype 2 HEV isolates which infect human only; Z group contains genotype 3 and genotype 4 HEV isolates which infect both human and animals. After analysis of amino acid sequences between ORF2 aa368 and aa606, four group-conserved sites that were all located in the neutralization region of ORF2 were identified. They are aa483, aa492, aa497 and aa599. Mutation analysis and capture PCR were then performed on these sites with a group of monoclonal antibodies. Results showed that the difference of the aa497 between H and Z groups was responsible for the maintenance of their group-specific immunodominant epitopes, probably through confirmation-dependent epitope changes. Thus, aa497 and its related change on the surface structure of HEV may play important roles in host selection by H and Z groups of HEV.
