Construction of recombinant fowlpox virus expressing E0 gene of classical swine fever virus shimen strain and the animal immunity experiment.
- Author:
Yang-Hui WANG
1
;
Pu-Hua LI
;
Miao-Tao ZHANG
;
Yan-Ming ZHANG
Author Information
1. College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, China. felin750@163.com
- Publication Type:Journal Article
- MeSH:
Animals;
Blotting, Western;
Chick Embryo;
Classical swine fever virus;
genetics;
immunology;
Enzyme-Linked Immunosorbent Assay;
Female;
Fowlpox virus;
genetics;
Mice;
Mice, Inbred BALB C;
Polymerase Chain Reaction;
Swine;
Vaccines, Synthetic;
immunology;
Viral Envelope Proteins;
genetics;
immunology;
Viral Vaccines;
immunology
- From:
Chinese Journal of Virology
2008;24(1):59-63
- CountryChina
- Language:Chinese
-
Abstract:
The CSFV E0 gene was amplified from the plasmid pMD18-T-E0 by PCR and cloned into the FPV-P11 and FPV-pSY. The identified recombinant DNA was transfected into chicken embryo fibroblasts (CEF) to package Fowlpox virus. E0 gene was confirmed to be integrated into the genome of recombinant Fowlpox virus by PCR, and Western blot was employed for detection of E0 expression in the chicken embryo fibroblasts infected with recombinant Fowlpox virus . The results of ELISA showed that systemic immune response to CSFV could be induced effectively after the mice were immunized three times with recombinant Fowlpox virus through celiac route, the titer of antibody was 1 : 4096. The protection experiment showed that 75% of piglets immunized three times with recombinant Fowlpox virus were survived, indicating that the recombinant Fowlpox virus was effective. This paper lays foundation for the study of CSFV live vector vaccine.
