The preliminary analysis of the recognition epitopes of anti-HEV monoclonal antibodies on HEV ORF2.
- Author:
Jun-Hui XIONG
1
;
Qing-Shun GUO
;
Sheng-Xiang GE
;
Ying GU
;
Yi-Xin CHEN
;
Ji MIAO
;
Hai-Lian DU
;
Wei-Guo SHI
;
Jun ZHANG
;
Ning-Shao XIA
Author Information
1. National Institute of Diagnostics and Vaccine Development of Infectious Disease, College of Life Science of Xiamen University, Xiamen 361005, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antibodies, Monoclonal;
immunology;
Enzyme-Linked Immunosorbent Assay;
Epitopes;
Hepatitis Antibodies;
immunology;
Hepatitis E virus;
immunology;
Mice;
Mice, Inbred BALB C;
Viral Proteins;
immunology
- From:
Chinese Journal of Virology
2008;24(2):83-87
- CountryChina
- Language:Chinese
-
Abstract:
Western blot, capture-PCR, blocking ELISA and synthetic polypeptides were used to systematically study the recognition epitopes on HEV ORF2 of 23 anti-HEV monoclonal antibodies(McAbs) which were previously generated in our laboratory directed against HEV ORF2. Results showed that seven McAbs recognized linear epitopes that located at aa408-458 of HEV ORF2 and 16 conformation-dependent McAbs, most of which recognized the surface epitopes of native HEV, located at aa459-606 of HEV ORF2. The systematical study of the recognition epitopes of anti-HEV McAbs on HEV ORF2 provides important information for the investigation of virus receptor and HEV infection mechanism, as well as its vaccine and diagnostics development.
