Preparation and identification of the monoclonal antibody specific to hemagglutinin of avian paramyxovirus type 2.
- Author:
Guo-Zhong ZHANG
1
;
Yu SONG
;
Yue-Lian CHEN
;
Ming WANG
;
Ji-Xun ZHAO
Author Information
1. College of Veterinary Medicine, China Agricultural University, Beijing 100094, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antibodies, Monoclonal;
biosynthesis;
immunology;
Avulavirus;
immunology;
Enzyme-Linked Immunosorbent Assay;
Hemagglutination Inhibition Tests;
Hemagglutinins, Viral;
immunology;
Mice;
Mice, Inbred BALB C
- From:
Chinese Journal of Virology
2008;24(2):148-151
- CountryChina
- Language:Chinese
-
Abstract:
A hybridoma cell line 1G4A7 secreting monoclonal antibody (McAb) specific to hemagglutinin of avian paramyxovirus type 2 (APMV-2) was developed by fusing the spleen cells of APMV-2 immunized BAlb/c mice with SP2/0 myeloma cells. The immunoglobulin subclass of 1G4A7 was IgG1 with light chain kappa and the affinity constant against APMV-2 was 1.02 X 10(10). Identified by HI and indirect ELISA, the McAb titers in ascities were 10 log 2 and 1 : 10(6) respectively. The McAb did not cross react with the common avian viruses, showing good specificity. There existed obvious differences in antigenitic relationship among APMV-2 viruses analyzed by HI and indirect ELISA using McAb 1G4A7.
